Marking and detection Alfa Aesar J64030, J64308, J64527, etc.

Labeling biomolecules with fluorophores allows researchers to detect certain components of biological systems. Fluorescence detection provides sensitivity in a variety of applications, from microscopy and imaging to flow cytometry and Western blotting. Fluorescent labeling is an effective method for bioanalytical purposes. Fluorophores can be easily attached to many peptides and proteins by conjugation of free thiols or amine side chains. For nucleic acids, the introduction of modified nucleotides with amino groups, thiol or other groups allows covalently attaching fluorescent probes to DNA and RNA chains. Some fluorophores can also be embedded in DNA strands, which facilitates their detection. Recently, click chemistry has become a convenient method of labeling biomolecules. It involves the reaction of a pair of reagents that are inert with respect to naturally occurring functional groups.

Models: J64030, J64308, J64527, J65668, J65127, J65577, J64892, J64859, J64948, J64549, J65637, J65377, J64128, etc.